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3 years ago

(a) The absorbance of mixture at 400 nm was 0.40.

Chemistry

1 answer:

laiz [17]3 years ago

6 0

Answer:

0.1 g/dl

Explanation:

The standard curve is a graph that relates the absorbance at 400 nm with the concentration of haemoglobin in mg/dl. To obtain the concentration from the absorbance value, we enter in the x-axis (absorbance at 400 nm) with the value 0.40 (the line between 0.2 and 0.6), we extrapolate the line to the curve and read the correspondent value on y-axis (concentration in mg/dl): 100 mg/dl.

So, we convert the concentration from mg/dl to g/dl by dividing into 1000:

100 mg/dl x 1 g/1000 mg = 0.1 g/dl

Therefore, the concentration of haemoglobin of the patient is 0.1 g/dl.

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3 years ago

What volumes of 0.200 M HCOOH and 2.00 M NaOH would make 500. mL of a buffer with the same pH as one made from 475 mL of 0.200 M

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36 ml of NaOh and 464 ml of HCOOH would be enough to form 500 ml of a buffer with the same pH as the buffer made with benzoic acid and NaOH.

What is benzoic acid found in?

Some natural sources of benzoic acid include: Fruits: Apricots, prunes, berries, cranberries, peaches, kiwi, bananas, watermelon, pineapple, oranges.
Spices: Cinnamon, cloves, allspice, cayenne pepper, mustard seeds, thyme, turmeric, coriander.

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With these values, we can calculate the volumes of NaOH and HCOOH needed to make the buffer.

NaOH volume

( 0.5 - 0.464)L

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HCOOH volume

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