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Answer:
Option 2, Half of the active sites are occupied by substrate
Explanation:
Michaelis-Menten expression for enzyme catalysed equation is as follows:
Here, is Michaelis-Menten constant and [S] is substrate concentration.
When [S]=Km
Rearrange the above equation as follows:
when [S]=Km, the rate of enzyme catalysed reaction becomes half of the maximum rate, that means half of the active sites are occupied by substrate.
Therefore, the correct option is option 2.