2 pts

During preparatory phase glucose is converted into fructose-1,6-bisphosphate. Till this time the carbon numbering remains the same i.e. if we will label carbon at 6th position of glucose, its position will remian the same in fructose-1,6-bisphosphate that means the labeled carbon will still remain at 6th position.

When fructose-1,6-bisphosphate is further catalyzed with the help of enzyme aldolase it is cleaved into two 3 carbon intermediates which are glyceraldehyde 3-phosphate (GAP) and dihyroxyacetone phosphate (DHAP). In this conversion, the first three carbons of fructose-1,6-bisphosphate become carbons of DHAP while the last three carbons of fructose-1,6-bisphosphate will become carbons of GAP. It simply means that GAP will acquire the last carbon of fructose-1,6-bisphosphate which is labeled. Now the last carbon of GAP which has phosphate will be labeled.

(2) Pay-off phase

During this phase, GAP is dehydrogenated into 1,3-bisphosphoglycerate (BPG) with the help of enzyme glyceraldehyde 3-phosphate dehydrogenase. This oxidation is coupled to phosphorylation of C1 of GAP and this is the reason why 1,3-bisphosphoglycerate has phosphates at 2 positions i.e. at position 1 in which phosphate is newly added and position 3rd which already had labeled carbon.

It is pertinent to mention here that BPG has a mixed anhydride and the bond at C1 is a very high energy bond. In the next step, this high energy bond is hydrolyzed into a carboxylic acid with the help of enzyme phosphoglycerate kinase and the final product is 3-phosphoglycerate. Hence, the carbon with low energy phosphate i.e. the carbon at 3rd position remains labeled.

3 0

3 years ago

In the process of making soap, I poured some of the cooked mixture through some muslin fabric, in order to separate the solid pa

lyudmila [28]

Answer:

filtering

Explanation:

you're pouring the mixture through muslin cloth to keep the particles and bigger peaces out of the soap.

6 0

3 years ago