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AveGali [126]
3 years ago
11

6. Салыстырып

Chemistry
1 answer:
Annette [7]3 years ago
7 0

Answer:

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Explanation:

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The molar absorptivity of a tyrosine residue at 280 nm is 2000 M-1cm-1, while for tryptophan it is 5500 M-1cm-1. A protein has b
adelina 88 [10]

Answer:

There are 4 tryptophans in the protein.

Explanation:

According to question,  protein contains one tyrosine residue and say x number of tryptophans.

Concentration of protein solution = 1.0 micromolar = 1.0\times 10^{-6} Molar

Molar absorptivity of a protein solution : \epsilon

\epsilon = \epsilon _{tyro}+\epsilon _{tryp}

=1\times 2000 M^{-1}cm^{-1}+x\times 5500 M^{-1}cm^{-1}

Length of the cuvette = l = 1.0 cm

Absorbance of protein solution at 280 nm = A = 0.024

A=\epsilon \times l\times c ( Beer-Lambert's law)

0.024=(1\times 2000 M^{-1}cm^{-1}+x\times 5500 M^{-1}cm^{-1})\times 1 cm\times 1.0\times 10^{-6} M

Solving for x :

x = 4

There are 4 tryptophans in the protein.

7 0
3 years ago
The symbol for gold is<br> a. go.<br> b. g.<br> c. au.<br> d. au.
Zolol [24]
Au is the symbol for gold. It comes from Latin word for gold, which is Aurunum. Its atomic number is 79. 
7 0
3 years ago
Read 2 more answers
What type of physical change is it when a liquid turns into a solid and a solid turns into a liquid
goblinko [34]

Answer:

Freezing and Melting (Fusion)

Explanation:

4 0
3 years ago
The pK1, pK2, and pKR of the amino acid lysine are 2.2, 9.1, and 10.5, respectively. The pK1, pK2, and pKR of the amino acid arg
almond37 [142]

Answer:

pH 9,8 is likely to work best for this separation

Explanation:

Ion exchange chromatography is a chemical process where molecules are separated by affinity to an ion exchange resin. To separate different aminoacids you must use the isoelectric point (That is the pH where the aminoacid will be in its neutral form).

For lysine, PI is:

pH = \frac{1}{2} (9,1+10,5) = 9,8

For arginine:

pH = \frac{1}{2} (9,0+12,5) = 10,75

At pH = 9,8 lysine will be in its neutral form and will not be retain in the column but arginine will be in +1 charge being retained by the ion exchange resin.

Thus, <em>pH 9,8 is likely to work best for this separation</em>

<em></em>

I hope it helps!

5 0
3 years ago
Draw the structural formula of the major product of the reaction of (S)-2,2,3-trimethyloxirane with MeOH, H . Use the wedge/hash
Katarina [22]

Answer:

(S)-3-methoxy-3-methylbutan-2-ol

Explanation:

In this case, we have an <u>epoxide opening in acid medium</u>. The first step then is the <u>protonation of the oxygen</u>. Then the epoxide is broken to generate the most <u>stable carbocation</u>. The nucleophile (CH_3OH) will attack the carbocation generating a new bond. Finally, the oxygen is <u>deprotonated</u> to obtain an ether functional group and we will obtain the molecule <u>(S)-3-methoxy-3-methylbutan-2-ol</u>.

See figure 1

I hope it helps!

8 0
3 years ago
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