Well, an independent variable ids the thing that stays the same and a dependent variable is the thing that changes.
At the most basic level, in order to produce oxygen and a carbohydrate (comprised of carbon, hydrogen, and oxygen), carbon dioxide must react with water as a source of hydrogen.
In order to construct carbohydrate molecules (often glucose) and release oxygen into the atmosphere, photosynthesis utilises carbon dioxide and water. Photosynthesis occurs in organelles called chloroplasts found in eukaryotic autotrophs like plants and algae.
Commonly used notation for photosynthesis is:
6CO
+ 6H
O C
H
O
+ 6O
This indicates that the reactants, six carbon dioxide molecules and six water molecules, are transformed into a sugar molecule and six oxygen molecules, the products, by light energy being absorbed by chlorophyll.
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Salt (not table salt!) is formed when an acid reacts with a base. Salt is a compound. Water is also produced as a by-product of this reaction.
Thin layer chromatography(TLC) works with the principle of separation through adsorption.
It is used in the isolation and extraction of lipids through the following steps:
- apply the lipid samples spots in the bottom of the plate.
- also apply sample solution to the marked spot
- pour the mobile phase into the TLC chamber and use a moist filter paper to cover it. this is done to maintain equal humidity.
- then place the plate in the TLC chamber and close it with a lid.
- the plate is immersed into the solvent (mobile phase) for its development. this is done, keeping in mind that the sample spot should be above the solvent.
- once the sample spots are developed, they are removed and dried.
- this is later viewed using the UV light chamber to see the isolation of the lipid sample.
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