Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
The correct matches are:
1. Exosphere - Temperatures reach as high as 2000 C yet it feels very cold
This is the top layer of the atmosphere. The atoms are so dispersed that despite it having very high temperature it doesn't feel like it at all.
2. Thermosphere - Particles that have enough energy can escape into space
The thermosphere is the fourth highest layer of the atmosphere. The atoms in this layer are relatively distant from one another, so the particles that have enough energy manage to escape easily into the exosphere and then the space.
3. Mesosphere - It is the coldest region of the atmosphere
The mesosphere is the third highest layer. In this layer the temperatures constantly drop, and they go down to -85 degrees, making it the coldest layer by far.
4. Stratosphere - Ninety percent ozone is in this layer
The startosphere has a separte zone in it which is dominated by only one gas, the ozone. It is called the ozone layer, the one that protects the Earth from too intense UV radiation, and in fact over 90% of this gas is locate here.
5. Troposphere - It is warm due to the heat from Earth's surface
The troposphere is the densest and lowest of the layers. It is the one that also has Greenhouse gases which manage to trap the heat that is radiated from the surface of the Earth, thus keeping this layer relatively warm.
Answer:
Almost everything in our surroundings represent chemical change
Explanation:
●Rusting of iron in presence of moisture and oxygen.
●Burning of wood.
●Milk becoming curd.
●Formation of caramel from sugar by heating.
●Baking of cookies and cakes.
●Cooking any food.
●Acid-base reaction.
●Digestion of food.
Here, we use the mole as we would use any other collective number: a dozen eggs; a Bakers' dozen; a Botany Bay dozen.
Of course, the mole specifies a much larger quantity, and if I have a mole of stuff then I have
6.022
×
10
23
individual items of that stuff. We can also specify an equivalent mass, because we also know the mass of a mole of iron, and a mole of oxygen etc........The mole is thus the link between the macro world of grams and kilograms and litres, that which we can measure out in the lab, to the micro world of atoms, and molecules, that which we can perceive only indirectly.
Here we have the formula unit
F
e
2
(
S
O
4
)
3
. If there is a mole of formula units, there are necessarily 2 moles of iron atoms, 3 sulfate ions,.......etc.
