Energy decreases as it moves uptrophic levels because energy is lost as metabolic heat when the organisms from one trophic level are consumed by organisms from the next level.Trophic level transfer efficiency (TLTE) measures the amount of energy that is transferred between trophic levels.
THIS IS NOT THE EXACT ANSWER BUT IT MIGHT HELP
The cover slips serves two purposes: (1) it protects the microscope's objective lens from contacting the specimen, and (2) it creates an even thickness (in wet mounts) for viewing.
Why is it always balloons?
anyways so the balloon volume goes somewhere else when it shrinks because the balloon is losing air i think