Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
the heat of reaction for a chemical reaction
How much is each gallon or how far are you going is the question you should be asking
<h3><u>Answer;</u></h3>
= 3032.15 kPa
<h3><u>Explanation;</u></h3>
Using the equation;
PV = nRT , where P is the pressure,. V is the volume, n is the number of moles and T is the temperature and R is the gas constant, 0.08206 L. atm. mol−1.
Volume = 7.5 L, T = 274 +273 = 547 K, N = 5 moles
Therefore;
Pressure = nRT/V
= (5 × 0.08206 × 547)/7.5 L
= 29.925 atm
But; 1 atm = 101325 pascals
Hence; Pressure = 3032150.63 pascals
<u>= 3032.15 kPa</u>
