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2 years ago

12

What is the difference between a pure solvent and a solution? Do they have the same physical properties?

1 answer:

pychu [463]2 years ago

5 0

Answer:

The physical properties of a solution are different from those of the pure solvent. ... Colligative properties are those physical properties of solutions of nonvolatile solutes that depend only on the number of particles present in a given amount of solution, not on the nature of those particles.

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MrRa [10]

Answer:

Explanation:

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2 years ago

The molar absorptivity of a tyrosine residue at 280 nm is 2000 M-1cm-1, while for tryptophan it is 5500 M-1cm-1. A protein has b

adelina 88 [10]

Answer:

There are 4 tryptophans in the protein.

Explanation:

According to question, protein contains one tyrosine residue and say x number of tryptophans.

Concentration of protein solution = 1.0 micromolar = $1.0\times 10^{-6} Molar$

Molar absorptivity of a protein solution : $\epsilon$

$\epsilon = \epsilon _{tyro}+\epsilon _{tryp}$

$=1\times 2000 M^{-1}cm^{-1}+x\times 5500 M^{-1}cm^{-1}$

Length of the cuvette = l = 1.0 cm

Absorbance of protein solution at 280 nm = A = 0.024

$A=\epsilon \times l\times c$ ( Beer-Lambert's law)

$0.024=(1\times 2000 M^{-1}cm^{-1}+x\times 5500 M^{-1}cm^{-1})\times 1 cm\times 1.0\times 10^{-6} M$

Solving for x :

x = 4

There are 4 tryptophans in the protein.

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Atom A has five protons, four neutrons, and five electrons. Atom

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