Answer:
Explanation: in exothermic reaction heat is released out
In endothermic reaction heat is absorbed
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The required mass of argon in given number of atoms of argon is 155.61 grams.
<h3>What is Avogadro's number?</h3>
Avogadro's number is the number of atoms which are present in 1 mole of any substance and it is equal to 6.022×10²³.
Relation between the mass and moles of any substance will be represented as:
- n = W/M, where
- W = given mass
- M = molar mass
So, moles of argon will be calculated by using given number of atoms as:
Moles = 2.4×10²⁴ atoms / 6.022×10²³ atoms per mole
Moles = 3.9 moles
Now mass will be:
W = (3.9mol)(39.9g/mol) = 155.61 g
Hence required mass of argon is 155.6g.
To know more about Avogadro's number, visit the below link:
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Answer:
There is two common methods to separate a phosphorylated protein from the proteins who does not have the phosphate group.
1. Western Blot test
The Western blot test is a method where we separate of the biological sample with SDS- PAGE (electrophoresis method) and then we transfer to a nitrocellulose membrane. We use a phospho-specific antibody to identify the protein of interest.
2. The ELISA test
this test is also a powerful method for measuring protein phosphorylation. Elisa test is more are quantitative than Western blot test and also shows great advantage in studies related with kinase activity. In this test we use a capture antibody specific for the desired protein, independent of the phosphorylation state. The target protein is binded to the antibody-coated plate. and finally a detection antibody specific for the phosphorylation site analyzed is added. In these test it is possible to use colorimetric or fluorometric detection.
