Answer:
A protein has four subunits whose molecular masses are 140, 80, and 60 kDa.
A disulfide bond links the two 80 kDa subunits (possibly identical).
Explanation:
Given that:
A protein has four subunits whose molecular masses are 140, 80, and 60 kDa.
A disulfide bond links the two 80 kDa subunits (possibly identical).
As a result of SDS and dithiothreitol analysis treatment, the molecular masses can not be 360 in total. They are 280, which implies that they are in short of 80 kDa. This means that there are possibilities that two groups with a molecular mass of 80 kDa which are joined by a disulfide bond.
The presence of SDS and dithiothreitol acts as a reducing agent, and they can break disulfide bonds whose pH is greater than 7, i.e. those in basic condition.
Answer:
If you contact water with a gas at a certain temperature and (partial) pressure, the concentration of the gas in the water will reach an equilibrium ('saturation') according to Henry's law.
Explanation:
This means: if you increase the pressure (e.g. by keeping the vial closed), the CO2 concentration will increase. So it simply depends what concentration you need for your assay: 'CO2-saturated' water at low pressure or 'CO2-saturated' water at high pressure.
Answer:
Pro exercise con suffication
Explanation:
...
