As the speed changed, what did you notice about the molecules’ freedom of movement? (amplify science)
1 answer:
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Answer:
At pH of 3.6, Peptide A and B will bind to the anion-exchange column, while neither peptide will bind to the cation-exchange column.
At pH of 6.6, Peptide A will bind to the cation-exchange column while Peptide B will bind to the anion-exchange column.
At pH of 10.0, Peptide A and B will bind to the cation-exchange column, whereas neither peptide will bind to the anion-exchange column.
<em>Note: The question is incomplete. A sample complete question is found in the attachment below.</em>
Explanation:
If pH is greater than pI, the net charge on the peptide is negative.
When pH = pI, the net charge is zero
When pH is less than pI, the net charge on the peptide is positive.
A positively charged peptide will bind to an anion-exchange column, whereas a negatively charged peptide will bind to a cation-exchange column.
At pH of 3.6, Peptide A and B will bind to the anion-exchange column, while neither peptide will bind to the cation-exchange column.
At pH of 6.6, Peptide A will bind to the cation-exchange column while Peptide B will bind to the anion-exchange column.
At pH of 10.0, Peptide A and B will bind to the cation-exchange column, whereas neither peptide will bind to the anion-exchange column.
