Mitochondria because is the power house for cell,site for respiration or energy release.
The answer is HCl, C3H6 and C2H5OH. Hydrochloric acid has the lowest boiling point among the three choices since it only has weak dipole-dipole and Van der Waal's forces between molecules which are much weaker than the forces of attraction present in propane and ethanol. Ethanol has the highest since the hydrogen bonding present in ethanol make it hard to break the bonds.
This is a guess, but I think it could be the lack of limiting factors.
If the hydrate gets too hot it could start splattering and cause a loss of sample.
