Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
exosphere and ionosphere.
Explanation:
very high up,the earth's atmosphere becomes very thin. the region where atoms and moleculesescape into space is referred to as the exosphere. exosphere is on top of the thermosphere.
scientists what is called the ionospherean extension of the thermosphere. so technically, ionosphere is not another atmospheric layer.
Elements in group 1-2, 13-18, the number of valence electrons is related to the group number. For example, in the first group, the alkali metals there is one valence electron, however in group 13, there are 3 valence electrons. Valence electrons are also known as the outershell electrons.
Answer:
A physical property is any property that is measurable, whose value describes a state of a physical system.
Explanation:
-color
-density
-volume
