Simply no.
And the problem wouldn't be only the lack of gravity, it would be also the lack of air.
Flying is an action not to far from swiming: something is moving trough some fluid. Of course that the fluid will incredibly matter, but the basics are the same.
When a eagle flies, it is pushing its wings against the air, and the air is holding that pressure against the wing, and, also for a bunch of anatomic reasons, the eagle is able to fly.
Without air and gravity, it will just keep floating around until one of the gravitational pulls wins the battle and attrack it to a painfull fate.
I hope I helped!