The most common pH indicator used in Simmons Citrate Agar is Bromthymol Blue (BTB)
Simmons Citrate Agar is a selective and differential medium used for the detection and differentiation of Enterobacteriaceae (gram-negative bacteria).
The medium contains sodium citrate as the sole carbon source, which is used to differentiate organisms based on their ability to utilize citrate as a sole carbon source.
The medium also contains pH indicators that change color based on the pH of the medium. The most common pH indicator used in Simmons Citrate Agar is Bromthymol Blue (BTB).
BTB is a pH indicator that turns yellow in acidic conditions and blue in basic conditions. As the bacteria metabolize the citrate in the medium, they produce acids, which cause the medium to become acidic.
This change in pH is detected by the BTB, which changes color from blue to yellow. The yellow coloration of the medium is an indication that the organism is utilizing citrate as a sole carbon source.
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