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Lana71 [14]
3 years ago
13

Describe how you could use two LB/agar plates, some E. coli, and some ampicillin to determine how E. coli cells are affected by

ampicillin.
Chemistry
1 answer:
KiRa [710]3 years ago
8 0

Answer:

For this experiment we are going to take plate 1 as the control plate, so, in it there will be just E. coli in LB/agar; in plate 2, we are going to put E. coli in LB/agar and some ampicillin.  Then, we have to wait for the E. coli colonies to form. After a while, the E. coli growth can be compared on both plates and determine if ampicillin affects or not the E. coli colonies.

Explanation:

If the ampicillin affects negatively E. coli colonies, we are going to observe that in plate 1 (control plate) there are E. coli colonies growing, but in plate 2, there is no E. coli colonies or, at least, there is a fewer number of colonies on it. If ampicillin doesn't affect E.coli, plate 1 (control) and plate 2 (ampicillin experiment) are going to be similar in number of colonies.

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Nh4cl is sometimes preferred instead of hcl or h2so4 for "acid" work-up after grignard reactions, particularly when the expected and desired product is a tertiary alcohol because NH4+ is a much milder acid than HCl or H2SO4, which achieve the protonation of

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