Answer:
The protein has 4 subunits: 2 subunits of 90 kDa, 1 subunit of 160 kDa and 1 subunit of 60 kDa
Explanation:
In gel electrophoresis, the SDS agent produces denaturation of the protein and confers negative charge, so the protein subunits can migrate according to their masses. It produces dissociation of the protein in its subunits but it cannot disrupt disulphyde bridges (S-S) that can bond subunits together.
From the data, with SDS we observe 3 bands ⇒ 180 kDa + 160 kDa + 60 kDa
The addition of dithiotreitol (DTT), a reducing agent, produces the disruption of disulphyde bridges. From the data:
With DTT ⇒ 160 kDa + 90 kDa + 60 kDa
We observe that 160 kDa and 60 kDa subunits are conserved (they are the same as with SDS only), but 180 kDa subunit is missing and in its place appears a band of 90 kDa - a half 180 kDa.
So, the band at 180 kDa is composed by two subunits bonded by a disulphyde bridge.
Therefore, the composition of the protein is: <em>1 subunit of 160 kDa, 2 subunits of 90 kDa and 1 subunit of 60 kDa</em>.
Answer:
There are 2 double bond units and 1 lone pair, which will try to get as far apart as possible - taking up a trigonal planar arrangement. Because the lone pair isn't counted when you describe the shape, SO2 is described as bent or V-shaped.
Explanation:
There are 2 double bond units and 1 lone pair, which will try to get as far apart as possible - taking up a trigonal planar arrangement. Because the lone pair isn't counted when you describe the shape, SO2 is described as bent or V-shaped.
Distillation is by definition separation by boiling point. Basically things that have lower boiling points will boil before things that have higher boiling points. so if you go into the temperature range between the two boiling points, one will boil and vaporize and one will remain a liquid (maybe solid).
Answer:
Nitrogen
Explanation:
Nitrogen has 5 Valence Electrons.
And its atomic mass is also less than Bismuth.
There are other elements in the 5th row but this one has the lowest atomic mass compared to the others.
Answer:
200J
Explanation:
This is because 500J - 300J = 200J
I hope this helps!!!
