Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.
Nitrate Reduction test:
- The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
- Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
- While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
- The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).
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hey there!:
A) Knowing theatre the protease is showing the highest activity at pH 4-6, implies that the amino acid that amino acid that it is acting in is an amino acid with a basic side chain. Therefore, the residues can be any one of the three basic amino acids being histidine, arginine or lysine , having basic side chains at neutral pH.
b) The mechanism of reaction of cysteine proteases is as follows:
First step in the reaction is the deprotonation of a thiol in the cysteine proteases's active site by an adjacent amino acid with a basic side chain, which might be a histidine residue. This is followed by a nucleophilic attack by the anionic sulfur of the deprotonated cysteine on the substrate carbonyl carbon.
Here, a part of the substrate is released with an amine terminus, restoring the His into a deprotonated form, thus forming a thioester intermediate, forming a link between the carboxy-terminal of the substrate and cysteine, resulting in thiol formation. Thus the name thiol proteases. The thioester bond is then hydrolyzed into a carboxylic acid moiety while again forming the free enzyme.
C) cysteine proteases have a pka of 8-9 but when they are deprotonated by a His residue, their pka would come down to 6-8, which would be their optimal pH for functioning. This is because there is a deprotonation of the thiol group , later restoring the HIS deprotonated form and then formation of a thioester bond. This thioester bond when hydrolysed will a carboxylate moeity , which is responsible for bringing the pH down towards a more acidic side.
d) at the optimal pH , the fraction of deprotonated cysteine and protonated B will be equal which will change with the change in pH.
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The answer is A. Voltaic cells couple oxidation and reduction half reactions to produce electromotive force from chemical potential energy. Electrolytic cells do the opposite in that they require electromotive force to store chemical energy.
The most abundant isotope among the given Isotopes will be Isotope B
<h3>How to calculate percentage abundance of isotopes ?</h3>
To calculate the percent abundance of each isotope in a sample of an element, we usually divide the number of atoms of a particular isotope by the total number of atoms of all isotopes of that element and then multiply the result by 100.
% Abundance = 15/50 x 100
= 30 %
% Abundance = 30/50 x 100
= 60 %
% Abundance = 5/50 x 100
= 10 %
Hence, The most abundant isotope among the given Isotopes will be Isotope B.
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Answer:5.5673
Explanation: I searched it up for you