Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
O.N. of Na = +1
O.N. of O = -2
Let, O.N. of Tin = x
1*2 + x + -2*2 = 0
2+x-4 = 0
x-2 = 0
x = 2
SO OPTION C IS YOUR ANSWER......
I think it’s light
Have fun :)
Answer:
The hotness or pungency of chilli is due to presence of a group of compounds called capsaicinoids. Among capsaicinoids, capsaicin and dihydrocapsaicin are present in major quantities, while homocapsaicin, nordihydrocapsaicin and homo-dihydrocapsaicin are present in very small quantities.
Answer:
a) ammonium ion
b) amide ion
Explanation:
The order of decreasing bond angles of the three nitrogen species; ammonium ion, ammonia and amide ion is NH4+ >NH3> NH2-. Next we need to rationalize this order of decreasing bond angles from the valence shell electron pair repulsion (VSEPR) theory perspective.
First we must realize that all three nitrogen species contain a central sp3 hybridized carbon atom. This means that a tetrahedral geometry is ideally expected. Recall that the presence of lone pairs distorts molecular structures from the expected geometry based on VSEPR theory.
The amide ion contains two lone pairs of electrons. Remember that the presence of lone pairs causes greater repulsion than bond pairs on the outermost shell of the central atom. Hence, the amide ion has the least H-N-H bond angle of about 105°.
The ammonia molecule contains one lone pair, the repulsion caused by one lone pair is definitely bless than that caused by two lone pairs of electrons hence the bond angle of the H-N-H bond in ammonia is 107°.
The ammonium ion contains four bond pairs and no lone pair of electrons on the outermost nitrogen atom. Hence we expect a perfect tetrahedron with bond angle of 109°.
