Answer:
Explanation:
For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.
Answer:
189.15cy
Explanation:
To understand this problem we need to understand as well the form.
It is clear that there is four wall, two short and two long.
The two long are 
The two long are 
The two shors are 
The height and the thickness are 14ft and 0.83ft respectively.
So we only calculate the Quantity of concrete,
![Q_c = [(2*122.08)+(2*86-375)]*14*0.833\\Q_c=4864.02ft^3](https://tex.z-dn.net/?f=Q_c%20%3D%20%5B%282%2A122.08%29%2B%282%2A86-375%29%5D%2A14%2A0.833%5C%5CQ_c%3D4864.02ft%5E3)
That in cubic yards is equal to 
Hence, we need order 5% plus that represent with the quantity

Answer:
center left-turn lane
Explanation:
A <em>center left turn lane</em> will be marked as described. The arrows, if present, generally indicate that left turns are permitted from the lane with these markings.
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If the double yellow lines are solid, they are considered to be a "barrier" and are not to be crossed.
Moisture content is measured in terms of pounds of water per pound of air (lb water/lb air) or grains of water per pound of air (gr. of water/lb air).
Hope this helps❤