Question

A student is quickly pipetting 129ul water and observes many bubbles in the tip. You as the second student demonstrate the correct order of steps:
1. In order to prevent bubbles, slowly let go off your thumb and release the pushbutton to aspirate.

2. Choose the correct pipette - P200 - and the correct tip to aspirate the correct amount of 129ul.

3. Push the pushbutton to the first stop before inserting the tip into the solution, and then put the tip in the solution tube.

4. Dial the volume on the adjustment ring by first over dialing the volume, and return to the correct amount of 129ul.

5. Push the pushbutton to the first stop, raise the tip above the liquid, and press it harder to the second step to discard 129ul.

6. Press the ejector to discard the tip.
Select one:
a. 2, 4, 3, 1, 5, 6
b. 2, 5, 4, 3, 1, 6
c. 6, 5, 4, 2, 3, 1
d. 1, 4, 2, 5, 3, 6
e. 2, 1, 5, 3, 4, 6

Answer 1

Push the pushbutton to the first stop before inserting the tip into the solution, and then put the tip in the solution tube.