Answer:
The answer is "
"
Explanation:
Given:
Molarity= number of moles
because it is 1 Liter

therefore,
it takes 20 mL of Tris.


So, take 
Answer:
A carbon–oxygen bond is a polar convalescent bond between carbon and oxygen. Oxygen has 6 valence electrons and prefers to either share two electrons in bonding with carbon, leaving the 4 nonbinding electrons in 2 lone pairs :O: or to share two pairs of electrons to form the carbon functional group.
I am confused on what your question is. Any more details?