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soldi70 [24.7K]
3 years ago
12

A homodimeric protein was found to migrate through SDS polyacrilamide gel electrophoresis (SDS-PAGE) with a mobility that matche

d that of a 45-kDa standard. What change in the experiment would increase the chances of observing the mobility expected for the 22.5-kDa monomer?A. Increasing the gel running timeB. Adding a reducing agentC. Using a higher voltageD. Removing the SDS
Physics
1 answer:
disa [49]3 years ago
5 0

Answer:

Adding a reducing agent

Explanation:

Homodimeric proteins are formed by non-covalent interactions between two identical proteins (monomers).

Some of these intermolecular interactions are mediated by disulphide bonds which can be broken by a  reducing agent like beta-mercaptoethanol (2-ME).

By adding the reducing agent to the gel, it is probable that the dimer is broken and the band observed will correspond to the two identical 22.5-kDa monomers.

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0/2 File Limit
slamgirl [31]

Answer:

Speed at which it will reach the ground is given as

v_f = 46.8 m/s

Total time for which it will remain in air is given as

t = 6.3 s

Explanation:

As we know that the object is projected upwards with speed

v_i = 15 m/s

g = - 9.81 m/s^2

now when it will reach the ground then we have

y = v_y t + \frac{1}{2} at^2

so we have

-100 = 15 t - \frac{1}{2}(-9.81) t^2

4.905 t^2 - 15 t - 100 = 0

so we have

t = 6.3 s

Now speed of the object when it reaches the ground is given as

v_f = v_i + at

v_f = -15 + (9.81)(6.3)

v_f = 46.8 m/s

8 0
3 years ago
Two airplanes leave an airport at the same time. The velocity of the first airplane is 730 m/h at a heading of 44.3 ◦ . The velo
Goshia [24]

Answer:

So airplane will be 1324.9453 m apart after 2.9 hour

Explanation:

So if we draw the vectors of a 2d graph we see that the difference in angles is  = 83 - 44.3 = 83-44.3=38.7^{\circ}

Distance traveled by first plane = 730×2.9 = 2117 m

And distance traveled by second plane = 590×2.9 = 1711 m

We represent these distances as two sides of the triangle, and the distance between the planes as the side opposing the angle 38.7.

Using the law of cosine, d^2 representing the distance between the planes, we see that:

d^2=2117^2 + 1711^2 -2\times (2117)\times (1711)cos(38.7)=1755480.2482

d = 1324.9453 m

4 0
3 years ago
A car accelerates ( speeds up) uniformly from 5 m/s * t * o 15 m/s in 2 seconds, calculate the car's acceleration?
Agata [3.3K]

Answer:

0.4 ms-²

Explanation:

v = u+ at

15 = 5+ a×25

10/5 = a

a = 0.4 ms-²

6 0
3 years ago
Give me an example of heterogeneous mixture
Dmitry [639]

Answer:

oil and water

Explanation:

7 0
3 years ago
Bobo, the clown, wants to set the world record for the furthest range traveled by a clown shot out of a cannon. Write a sentence
Furkat [3]

Solution:

The world record for the longest flight by a human cannonball is approximately 200 feet and therefore the human cannon catapult that Bobo would need is one that would be able to propel him with an initial velocity of more than 120 kilometer per hour (more than the speed of the current record holder) with the cannon directed at an angle 45 degrees above the horizonal

Learn more about the projectile motion here:

brainly.com/question/24240176

5 0
3 years ago
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