1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
soldi70 [24.7K]
3 years ago
12

A homodimeric protein was found to migrate through SDS polyacrilamide gel electrophoresis (SDS-PAGE) with a mobility that matche

d that of a 45-kDa standard. What change in the experiment would increase the chances of observing the mobility expected for the 22.5-kDa monomer?A. Increasing the gel running timeB. Adding a reducing agentC. Using a higher voltageD. Removing the SDS
Physics
1 answer:
disa [49]3 years ago
5 0

Answer:

Adding a reducing agent

Explanation:

Homodimeric proteins are formed by non-covalent interactions between two identical proteins (monomers).

Some of these intermolecular interactions are mediated by disulphide bonds which can be broken by a  reducing agent like beta-mercaptoethanol (2-ME).

By adding the reducing agent to the gel, it is probable that the dimer is broken and the band observed will correspond to the two identical 22.5-kDa monomers.

You might be interested in
24. An elevator is moving vertically up with an acceleration a. The force exerted on the floor by a passenger of mass m is
Wewaii [24]

<u>Given </u><u>:</u><u>-</u>

  • An elevator is moving vertically up with an acceleration a.

<u>To </u><u>Find</u><u> </u><u>:</u><u>-</u>

  • The force exerted on the floor by a passenger of mass m .

<u>Solution</u><u> </u><u>:</u><u>-</u>

As the man is in a accelerated frame that is <u>non </u><u>inertial</u><u> frame</u><u> </u>, we would have to think of a pseudo force .

  • The direction of this force is opposite to the direction of acceleration the frame and its magnitude is equal to the product of mass of the concerned body with the acceleration of the frame .

For the FBD refer to the attachment . From that ,

\implies Weight_{apparent}= mg + ma

<u>Hence</u><u> </u><u>option</u><u> </u><u>d </u><u>is </u><u>correct</u><u> </u><u>choice </u><u>.</u>

<em>I </em><em>hope</em><em> this</em><em> helps</em><em> </em><em>.</em>

3 0
2 years ago
A particle with charge -40.0nC is on the x axis at the point with coordinate x=0 . A second particle, with charge -20.0 nC , is
ICE Princess25 [194]

A particle with charge -40.0nC is on the x axis at the point with coordinate x=0 . A second particle, with charge -20.0 nC, is on the x axis at x=0.500 m.

No, there is no point at a finite distance where the electric potential is zero.

Hence, Option D) is correct.

What is electric potential?

Electric potential is the capacity for doing work. In the electrical case, a charge will exert a force on some other charge and the potential energy arises. For example, if a positive charge Q is fixed at some point in space, any other positive charge when brought close to it will experience a repulsive force and will therefore have potential energy.

It is also defined as the amount of work required to move a unit charge from a reference point to a specific point against an electric field.

To learn more about electric potential, refer to:

brainly.com/question/15764612

#SPJ4

4 0
1 year ago
A 3.5 kg object moving in two dimensions initially has a velocity v1 = (12.0 i^ + 22.0 j^) m/s. A net force F then acts on the o
lys-0071 [83]

Answer:

The work done by the force is 820.745 joules.

Explanation:

Let suppose that changes in potential energy can be neglected. According to the Work-Energy Theorem, an external conservative force generates a change in the state of motion of the object, that is a change in kinetic energy. This phenomenon is describe by the following mathematical model:

K_{1} + W_{F} = K_{2}

Where:

W_{F} - Work done by the external force, measured in joules.

K_{1}, K_{2} - Translational potential energy, measured in joules.

The work done by the external force is now cleared within:

W_{F} = K_{2} - K_{1}

After using the definition of translational kinetic energy, the previous expression is now expanded as a function of mass and initial and final speeds of the object:

W_{F} = \frac{1}{2}\cdot m \cdot (v_{2}^{2}-v_{1}^{2})

Where:

m - Mass of the object, measured in kilograms.

v_{1}, v_{2} - Initial and final speeds of the object, measured in meters per second.

Now, each speed is the magnitude of respective velocity vector:

Initial velocity

v_{1} = \sqrt{v_{1,x}^{2}+v_{1,y}^{2}}

v_{1} = \sqrt{\left(12\,\frac{m}{s} \right)^{2}+\left(22\,\frac{m}{s} \right)^{2}}

v_{1} \approx 25.060\,\frac{m}{s}

Final velocity

v_{2} = \sqrt{v_{2,x}^{2}+v_{2,y}^{2}}

v_{2} = \sqrt{\left(16\,\frac{m}{s} \right)^{2}+\left(29\,\frac{m}{s} \right)^{2}}

v_{2} \approx 33.121\,\frac{m}{s}

Finally, if m = 3.5\,kg, v_{1} \approx 25.060\,\frac{m}{s} and v_{2} \approx 33.121\,\frac{m}{s}, then the work done by the force is:

W_{F} = \frac{1}{2}\cdot (3.5\,kg)\cdot \left[\left(33.121\,\frac{m}{s} \right)^{2}-\left(25.060\,\frac{m}{s} \right)^{2}\right]

W_{F} = 820.745\,J

The work done by the force is 820.745 joules.

6 0
3 years ago
X = 20 <br> v = 3.5 m/s <br> t = ?
N76 [4]

Answer:

16.5 I think

Explanation:

3 0
3 years ago
Which statements are true about ionic compounds?A. An ionic compound can be named using prefixes.B. An ionic compound is made of
Damm [24]
The correct answer is d.
5 0
3 years ago
Other questions:
  • A computer technician always touches the metal body of a computer before touching any of its electrical parts. why?
    15·1 answer
  • A system gains 757 kJ757 kJ of heat, resulting in a change in internal energy of the system equal to +176 kJ.+176 kJ. How much w
    13·1 answer
  • What is 100N in kilograms
    13·2 answers
  • Light passes straight through two polarizing filters in which the axis of polarization of the second filter is rotated 45 degree
    9·1 answer
  • What do hydroelectric plants use to generate electrical energy?
    7·2 answers
  • Give you point but please i need help in physcis
    7·1 answer
  • Explain the role of convection currents In the formation of sea or land breazes
    10·1 answer
  • Baggage on the floor of the baggage car of a high-speed train is not prevented from moving other than by friction. The train is
    13·1 answer
  • 3. The pressure at the bottom of the ocean is great enough to crush submarines with steel walls that are 10 centimeters thick. S
    15·2 answers
  • How does reflection differ from refraction and diffraction?
    7·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!