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soldi70 [24.7K]
3 years ago
12

A homodimeric protein was found to migrate through SDS polyacrilamide gel electrophoresis (SDS-PAGE) with a mobility that matche

d that of a 45-kDa standard. What change in the experiment would increase the chances of observing the mobility expected for the 22.5-kDa monomer?A. Increasing the gel running timeB. Adding a reducing agentC. Using a higher voltageD. Removing the SDS
Physics
1 answer:
disa [49]3 years ago
5 0

Answer:

Adding a reducing agent

Explanation:

Homodimeric proteins are formed by non-covalent interactions between two identical proteins (monomers).

Some of these intermolecular interactions are mediated by disulphide bonds which can be broken by a  reducing agent like beta-mercaptoethanol (2-ME).

By adding the reducing agent to the gel, it is probable that the dimer is broken and the band observed will correspond to the two identical 22.5-kDa monomers.

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In a series rlc ac circuit, a second resistor is connected in parallel with the resistor previously in the circuit. as a result
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According to the given statement:

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<h3>The definition of series circuits:</h3>

electrical circuit. The path that the entire current takes as it passes through each component makes up a series circuit. Branching is used in parallel circuits to divide the current and limit the amount that flows through each branch.

<h3>How does a series circuit operate?</h3>

According to this definition, there are three principles of series circuits: all parts share the same current, resistances add up to a larger total resistance, and voltage drops add up to a larger total voltage. In the definition of a series circuit, all of these guidelines have their origin.

<h3>According to the given information:</h3>

The impedance of a series circuit is

       Z₀² = R² + (X_L-X_C) ²

The initial resistance impedance shifts to when we add another resistor to the series

              Z² = (R + R₂) ² + (X_L - X_C) ²

Let's examine this sentence.

  • The frequency response remains unchanged, which is the first thing we notice.
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           Z = R + R₂

To know more about electrical circuit visit:

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6 0
1 year ago
The existence of the dwarf planet Pluto was proposed based on irregularities in Neptune's orbit. Pluto was subsequently discover
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Distance between Neptune and Pluto, r=4.5\times 10^{12}\ m

The force of gravity is balanced by the gravitational force between Neptune and Pluto. It is given by :

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So, the acceleration due to gravity at Neptune due to Pluto is 4.61\times 10^{-14}\ m/s^2. Hence, this is the required solution.  

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