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soldi70 [24.7K]
3 years ago
12

A homodimeric protein was found to migrate through SDS polyacrilamide gel electrophoresis (SDS-PAGE) with a mobility that matche

d that of a 45-kDa standard. What change in the experiment would increase the chances of observing the mobility expected for the 22.5-kDa monomer?A. Increasing the gel running timeB. Adding a reducing agentC. Using a higher voltageD. Removing the SDS
Physics
1 answer:
disa [49]3 years ago
5 0

Answer:

Adding a reducing agent

Explanation:

Homodimeric proteins are formed by non-covalent interactions between two identical proteins (monomers).

Some of these intermolecular interactions are mediated by disulphide bonds which can be broken by a  reducing agent like beta-mercaptoethanol (2-ME).

By adding the reducing agent to the gel, it is probable that the dimer is broken and the band observed will correspond to the two identical 22.5-kDa monomers.

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Answer:

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Correction

A student measures the mass <em><u>8cm3</u></em> block of brown sugar to be 12.9g. what is the density of the brown sugar

Answer:

1.6\ g/cm^{3}

Explanation:

Density is defined as mass per unit volume of an object expressed as \rho=\frac {m}{v} where \rho is the density, m is the mass of sugar and v is the volume of the sugar. Considering that the volume is given as 8cm3 for sugar then we substitute this for v and mass of 12.9 g we substitute for g then the density will be

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Answer:

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Explanation:

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