B. The answer is: All nucleotides have a phosphorus atom that can be replaced with 32P.
Nucleotides contain a nitrogenous base, a five-carbon sugar, and, at least, one phosphate group. Exactly that phosphate group in the nucleotide has the phosphorus atom. Therefore, the phosphorus atom in the nucleotide can be replaced with radioactive phosphorus-32 (32P).
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Answer:
The first and last choice
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Joseph will not get the correct results for his samples. The spectophotometer will measure wrong absorbance values for the sample. It is highly advised to callibrate the instrument by first setting the absorbance of the solvent to zero. After it is done, only then one must determine the absorbance of the test solutions.