Biuret reagent will indicate the presence of protein in a given sample. It is also known as the Piotrowski's test. This reagent consists of copper (II) sulfate and sodium hydroxide. It detects peptide bonds by the reaction of the copper ions in an alkaline solution. The copper ions would form violet colored complexes when peptide is present in the solution. From this test, concentration can be calculated since the intensity of the color depends on the amount of peptide bonds and according to the Beer-Lambert law concentration and the absorption of light is proportional. The concentration is calculated by a spectrophotometric technique at a wavelength of 540 nm.
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The chemical formula C₂H₆O, which is designated as option D, is equivalent to this structural formula.
<h3><u>What is a Chemical Formula ?</u></h3>
Chemical element symbols, numbers, and occasionally additional symbols like parentheses, dashes, brackets, commas, and plus (+) and minus () signs are used in a chemical formula to represent information about the chemical proportions of the atoms that make up a certain chemical compound or molecule.
- An empirical formula represents by symbols, such as Na for sodium and Cl for chlorine, with subscripts that show the relative number of atoms in each.
- The composition of any member of an entire class of compounds can be represented by a general formula, a sort of empirical formula.
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It is a trial aimed to reduce bias during an experiment. An example would be a sugar pill, something that has no real effect so that the results of the true trial can accurately be compared. Its like a control group.
