Answer:
0.1 M
Explanation:
The following data were obtained from the question:
Initial volume (V1) = 100 mL
Initial concentration (C1) = 0.5 M
Final volume (V2) = 500 mL
Final concentration (C2) =?
Using the dilution formula C1V1 = C2V2, the new concentration of the solution can be obtained as follow:
C1V1 = C2V2
0.5 × 100 = C2 × 500
50 = C2 × 500
Divide both side by 500
C2 = 50/500
C2 = 0.1 M
Therefore, the new concentration of the solution is 0.1 M
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Answer:
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Answer:
25 mM Tris HCl and 0.1% w/v SDS
Explanation:
A <em>10X solution</em> is ten times more concentrated than a <em>1X solution</em>. The stock solution is generally more concentrated (10X) and for its use, a dilution is required. Thus, to prepare a buffer 1X from a 10X buffer, you have to perform a dilution in a factor of 10 (1 volume of 10X solution is taken and mixed with 9 volumes of water). In consequence, all the concentrations of the components are diluted 10 times. To calculate the final concentration of each component in the 1X solution, we simply divide the concentration into 10:
(250 mM Tris HCl)/10 = 25 mM Tris HCl
(1.92 M glycine)/10 = 0.192 M glycine
(1% w/v SDS)/10 = 0.1% w/v SDS
Therefore the final concentrations of Tris and SDS are 25 mM and 0.1% w/v, respectively.
