Answer:
Cells are the smallest living unit of an organism
A foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction.
<h3>How to explain the reaction?</h3>
With the aid of two enzymes, ligase and restriction enzymes, a foreign DNA molecule can be incorporated into a bacterial plasmid during a transformation reaction. Each enzyme detects a target DNA sequence and cuts it nearby, while ligase aids in connecting the DNA. When two bits of DNA have complimentary bases, it facilitates their joining.
Plasmid and the insert fragment are both present in the microfuge tube, and they both have compatible sticky ends. However, the ligase has been denatured and is no longer active because the prior student left it outside rather than freezing it; despite this, we had already put the ligase into the tube. Ligase aids in binding the plasmid and insert fragments together, but because it is denatured in this instance, it will no longer be able to do so. As a result, no transformation process will take place. And since ligase links DNA fragments together by catalyzing the development of connections between the nearby nucleotides, the two fragments will not be able to unite.
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When heated, particles vibrate faster, thus increasing the distance between one another. The distance between these particles results in changes of state. Therefore, increased molecular motion results in expansion of an object. This works vice versa for cooling. As the vibrations slow down, the particles become closer together. This results in contraction.
