Explain the use of a vacuum gauge.
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Answer:
The rate of cell metabolism is limited by mass transfer since the value of maximum cell concentration obtained (38 g/l) is lower than 50 g l-1, the value planed.
Explanation:
Data
<u>kLa</u> = 0.17/s
<u>Solubility of oxygen</u> = 8 × 10^-3 kg / m^3
<u>The maximum specific oxygen uptake rate </u>= 4 mmol O2 / g h.
<u>Concentration of oxygen</u> = 0.5 × 10^-3 kg/ m^3
<u>**The maximum cell density</u> = 50 g/l
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The calculated maximum cell concentration:
xmax= kLa · CAL*/ qo
CAL* is the solubility of oxygen in the broth and qo is the specific oxygen uptake rate
Replacing the data given
xmax= ( 0.17/s ) · (8 × 10^-3 kg / m^3) / 4 mmol O2 / g h
4 mmol O2 / g h to kg O2/ g s
= 3.56 x 10^-3 kg O2/ g s
So then,
xmax= ( 0.17/s ) · (8 × 10^-3 kg / m^3) / 3.56 x 10^-3 o kg O2/ g s
xmax= 3. 8 x 10^4 g/ m^3 = 38 g/l
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