Answer:
The correct/closest option is b
Explanation:
Restriction enzymes are enzymes (endonucleases) that cut short DNA strands at specific sites. Hence, each restriction enzyme has it's own specific site (between two bases) it cuts at. There are two types of end that can be produced by this cut; the blunt end and the sticky end.
A restriction enzyme recognizes (palindromic sequence) and cut in it's own specific end.
For example, if a restriction enzyme cuts between a guanine (G) and an adenine (A), and it cuts a palindromic double stranded DNA in the manner below, it produces a sticky end.
G║AATTC
CTTAA║G
And if a restriction enzyme cuts between guanine (G) and cytosine (C) in the manner below, it produces a blunt end.
GGG║CCC
CCC║GGG
Hence, from the question, restriction enzymes (although chosen by the scientist based on desired sequence to be cut) recognize the sticky or blunt ends itself.
Answer:
Explanation:
For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.
Explanation:
Clearance:
For easy matching and dis matching of hole and shaft we use size of hole little bit more than the size of shaft and this difference in size is called clearance.
Backlash:
It is the clearance between the two mating gear to avoids failure of gears.Actually when temperature of gears increases then at the same time the size of gear also increases ,due this there is a possibility foe jamming of gears so to avoids this backlash is provides.
Interference:
When two gears are matting then addendum of one gear inters into the deddendum of another gear and due to this gears get jam .This phenomenon is called interference.
Technician a is correct because he says that Many common rail injectors filters can be bypassed by dirt, which can lead to an injector sticking open and continuously fueling a cylinder.
Coalescence is used to separate the water and fuel. To the fuel injector cleaning kit, fasten your air compressor. Diesel engines run at compression ratios that are greater than those of gasoline engines. greater ratio compared to gasoline engines. increased thermal expansion as a result. more fuel energy that is transformed into usable power. The great benefit of using a dry cylinder sleeve is that by quickly installing new sleeves, the cylinder block can be quickly restored to its original specifications. Vacuum drying can be used to get rid of small amounts of water. A nozzle is used to spray the fuel into the vacuum chamber of engines. Air and unsolved free water are taken out of the oil. The fuel is evenly dispersed, which facilitates efficient drying.
Learn more about injectors here:
brainly.com/question/27969202
#SPJ4
