Tris and its conjugate base would be the best buffer at pH 8.
Further Explanation:
Buffer solution:
The solutions that oppose any change in their pH on addition of small amounts of acid or base are called buffer solutions. These are formed wither by a weak base and its conjugate acid or a weak acid and its conjugate base.
Henderson-Hasselbalch equation for a buffer solution is as follows:
…… (1)
A buffer is most effective when the concentration of both acid and its conjugate base is the same. So equation (1) then modifies as follows:
…… (2)
Therefore a buffer works best if its pH becomes equal to
.
The formula to calculate
is as follows:
…… (3)
Here,
is the dissociation constant of acid.
Substitute
for
in equation (3) to calculate
of tris.

Substitute
for
in equation (3) to calculate
of MES.

Substitute
for
in equation (3) to calculate
of acetic acid.
Substitute
for
in equation (3) to calculate
of formic acid.

Substitute
for
in equation (3) to calculate
of HEPES.

The value of
of tris is nearly equal to the required pH. So tris along with its conjugate base would be the best buffer at pH 8.
Learn more:
Write the chemical equation responsible for pH of buffer containing and : brainly.com/question/8851686
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Answer details:
Grade: High School
Chapter: Acid, base and salts
Subject: Chemistry
Keywords: tris, acetic acid, formic acid, MES, HEPES, pH, pKa, Henderson, best buffer, 7.49, 8.20, 4.74, 3.74, Ka.