Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
Answer:
b. potassium.
Explanation:
Potassium-sparing diuretics and salt substitutes are diuretics that eliminate salt and water but save potassium. They act by inhibiting the conducting sodium channels in the collecting tubule, such as amiloride and triamterene, or by blocking aldosterone, such as spironolactone.
Concomitant use of potassium-sparing diuretics together with salt substitutes may result in dangerously high blood levels of serum potassium. For this reason, it is important to consult a physician before taking these substances at the same time to avoid potential problems with potassium accumulation.
Answer:
C. Condensation
Explanation:
When molecules release heat energy, it is called exothermic. In this case, condensation would be an example of this.
Answer:
The relationship of the speed of sound, its frequency, and wavelength is the same as for all waves: vw = fλ, where vw is the speed of sound, f is its frequency, and λ is its wavelength. ... The more rigid (or less compressible) the medium, the faster the speed of sound
Explanation:
