Question

You will need to prepare 12 mL of 25% Sodium Phosphate Buffer (pH 4) solution for Activity 2. What volume of the stock Sodium Phosphate Buffer and Water will you need to measure out

Answer 1

Assuming the concentration of stock solution is 50% sodium phosphate buffer solution, the volume of stock solution required is 6 mL and the volume of water required is 6 mL.

What volume of a stock Sodium phosphate buffer and water is needed to 12 mL of 25% sodium phosphate buffer of pH 4?

The process of preparing solutions from stock solutions of higher concentration is known as dilution.

Dilution is done with the aid of the dilution formula given below:

• C1V1 = C2V2

where

• C1 is the concentration of stock solution
• V1 is the volume of stock solution required to prepare a diluted solution
• C2 is the concentration of the diluted solution prepared
• V2 is the final volume of the diluted solution

From the data provided:

C1 is not given

V1 is unknown

C2 = 25%

V2 = 12 mL

• Assuming C1 is 50% solution

Volume of stock, V1, required is calculated as follows:

V1 = C2V2/C1

V1 = 25 × 12 /50

V1 = 6 mL

Therefore, the volume of stock solution required is 6 mL and the volume of water required is 6 mL.

Learn more about dilution formula at: brainly.com/question/7208546